High efficiency transfection based on low electric field...

Details High efficiency transfection based on low electric field... High efficiency transfection based on low electric field...

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2377565

A method is provided for introducing nucleic acid into a cell, by contacting the cell with a nucleic acid and applying a low electrical field impulse for a long pulse length. A method is provided for introducing a polypeptide into a cell, by contacting the cell with the polypeptide and applying a low electrical field impulse for a long pulse length.

L'invention concerne un procédé permettant l'introduction d'acide nucléique dans une cellule par la mise en contact de la cellule avec un acide nucléique et l'application d'une impulsion de faible intensité de champ électrique pendant une longue durée d'impulsion. L'invention concerne aussi un procédé permettant l'introduction d'un polypeptide dans une cellule par la mise en contact de la cellule avec le polypeptide et l'application d'une impulsion de faible intensité de champ électrique pendant une longue durée d'impulsion.

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