Methods for rapid microbial detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01) C12N 1/21 (2006.01) C12N 15/03 (2006.01) C12N 15/52 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2066561

A method of testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; kill- ing extracellular bacteriophage without at the same time killing phage- infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bac- teria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria.

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