Synthetic protein folding catalysis

Details Synthetic protein folding catalysis Synthetic protein folding catalysis

C - Chemistry, Metallurgy

07

K

C07K 1/113 (2006.01) C12N 9/02 (2006.01) C12N 9/90 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2260795

An analysis of the protein folding characteristics of the eukaryotic protein folding enzyme protein disulfide isomerase (PDI) led to a study of the minimally sufficient motif for catalytic activity of that enzyme as well as the prokaryotic enzyme thioredoxin. Based on such study, a model for this catalytic activity was developed which was used to predict what non-protein catalysts might substitute for this enzymatic activity. Based on this analysis, it was predicted that a small molecular weight dithiol molecule having a pK a of less than about 8.0 and an E~' of more than about-0.25V could catalyze the formation of proper disulfide bonds in a eukaryotic protein. Subsequently, it was verified that such a dithiol, such as the exemplary molecule BMC, is capable of catalyzing the proper formation of disulfide bonds, and the proper folding of proteins, both in vivo and in vitro. This permits a small organic molecule to be substituted for an enzymatic system in protein synthesis.

Une analyse des caractéristiques de plicature des protéines de l'enzyme eucaryote de plicature des protéines, la protéine disulfure isomérase (PDI), a abouti à l'étude du motif minimal suffisant pour l'activité catalytique de ladite enzyme, ainsi que d'une enzyme procaryote, la thioredoxine. Sur la base de cette étude, un modèle de cette activité catalytique a été développé et utilisé pour prédire quels catalyseurs non protéiques pouvaient se substituer à ladite activité enzymatique. Sur la base de cette analyse, il a été prédit qu'un dithiol de faible poids moléculaire, ayant un pKa inférieur à 8,0 environ et un E DEG ' supérieur à -0,25 V environ, pouvait catalyser la formation de ponts disulfure corrects dans une protéine eucaryote. A partir de là, il a été vérifié qu'un dithiol de ce type, tel que la moléculaire BMC prise pour exemple, était capable de catalyser la formation correcte des ponts disulfure et la plicature correcte des protéines, aussi bien in vivo qu'in vitro. Ceci a permis, dans la synthèse protéique, de remplacer un système enzymatique par une petite molécule organique.

Affiliated with

Also associated with

Rating

Synthetic protein folding catalysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthetic protein folding catalysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic protein folding catalysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1585930