Artificial stimulation process of cells and ovocytes culture...

Details Artificial stimulation process of cells and ovocytes culture... Artificial stimulation process of cells and ovocytes culture...

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) A61D 19/02 (2006.01) C12M 1/42 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2078138

The present invention concerns a process for artificially stimulating a group of cells reproducing the effect of the biochemical regulation mechanism, controlled in natural physiological conditions by an internal oscillator, comprising the steps below: a) the cells are placed in in vitro culture for a given time; b) the ells are placed in the pulsing medium after the culture medium has been removed; c) the cells are subjected to pulses generated by an electric field; d) the cells are once more placed in the culture medium; e) the previous stages are repeated a certain number of times; f) cells in a uniform active state are obtained. It also concerns a device for the culture of ovocytes facilitating the operation of the process.

PRECIS DE DIVULGATION La présente invention concerne un procédé de stimulation artificielle d'un ensemble de cellules reproduisant l'effet d'un mécanisme de régulation biochimique, contrôlé dans les conditions physiologiques naturelles par un oscillateur interne, caractérisé en ce qu'il comprend les étapes suivantes : a) les cellules sont placées en culture in vitro pendant un temps déterminé, b) les cellules sont placées dans le milieu d'impulsion, après élimination du milieu de culture, c) on soumet les cellules à une impulsion générée par un champ électrique, d) les cellules sont à nouveau placées dans le milieu de culture, e) on répète les étapes précédentes un certain nombre de fois, f) on obtient des cellules dans un état activé homogène. Elle concerne également un dispositif de culture d'ovocytes permettant la mise en oeuvre du procédé.

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