Lipases with improved surfactant resistance

Details Lipases with improved surfactant resistance Lipases with improved surfactant resistance

C - Chemistry, Metallurgy

12

N

C12N 9/20 (2006.01) C11D 3/386 (2006.01) C12N 1/21 (2006.01) C12N 9/18 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2189441

The present invention provides mutant lipases which retain lipase activity, but have improved surfactant resistance and are thus highly advantageous for use in detergent compositions. Preferred lipases of this type include modified functional forms of a lipase of Pseudomonas alcaligenes having one or more amino acid substituents at or near the surface which effect interaction of the hydrophobic portions of surfactant molecules with initiation sites on the enzyme for surfactant denaturation.

Lipases mutées qui conservent leur activité de lipase, mais possèdent une résistance aux tensioactifs améliorée et conviennent donc particulièrement à l'utilisation dans des compositions détergentes. Les lipases préférées de ce type comprennent des formes fonctionnelles modifiées d'une lipase de Pseudomonas alcaligenes ayant un ou plusieurs substituants acides aminés au niveau ou à proximité de la surface, ce qui modifie l'interaction des parties hydrophobes des molécules de tensioactif avec des sites sur l'enzyme pour la dénaturation par le tensioactif.

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