C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/04 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 15/00 (2006.01) C12P 21/04 (2006.01)
Patent
CA 2463905
A method for formulating a glucose oxidase enzyme with peroxide resistant properties and a glucose oxidase enzyme formulated by the method. The enzyme formulation method results in a glucose oxidase enzyme with improve resistance to peroxide, and therefore, with improved resistance to oxidative inactivation. The method employs directed evolution techniques to evolve glucose oxidase to achieve the desirable properties. A peroxide resistant glucose oxidase may improve the longevity of, for example, glucose biosensors in which a peroxide resistant glucose oxidase may be placed.
L'invention concerne un procédé de formulation d'enzyme glucose-oxydase à propriétés de résistance au peroxyde, et une enzyme résultante. Ce procédé permet de formuler une enzyme glucose-oxydase résistant mieux au peroxyde, et résistant donc mieux à l'inactivation oxydative. Dans le cadre du procédé, on utilise des techniques d'évolution dirigée qui induisent une évolution de la glucose-oxydase vers les propriétés recherchées. Une glucose-oxydase résistant au peroxyde peut améliorer, par exemple, la longévité de biocapteurs de glucose dans lesquels on établit éventuellement cette glucose-oxydase.
Reghabi Bahar
Shah Rajiv
Medtronic Minimed Inc.
Oyen Wiggs Green & Mutala Llp
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