Heteroduplex pcr cloning

Details Heteroduplex pcr cloning Heteroduplex pcr cloning

C - Chemistry, Metallurgy

12

N

C12N 15/66 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2265241

The invention relates to a method of cloning a target nucleic acid sequence by performing two PCR reactions to generate two FCR products differing in terminal sequences. The PCR products thus obtained are denatured and annealed together to form a heteroduplex, which is ready for ligation and transformation of bacteria without ay restriction digestion.

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