Thermostablization of dna polymerase by protein folding...

Details Thermostablization of dna polymerase by protein folding... Thermostablization of dna polymerase by protein folding...

C - Chemistry, Metallurgy

12

N

C12N 9/96 (2006.01) C07K 1/00 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C07K 14/195 (2006.01)

Patent

CA 2662024

The present invention relates to maintaining the activity and stability of enzymes and biologically active proteins at increased temperatures by contacting same with a combination of isolated passive and active chaperones from a hyperthermopilic Archaeon, wherein the chaperones may include heat shock proteins, prefoldin and/or chaperonin proteins.

L'invention concerne le maintien de l'activité et de la stabilité d'enzymes et de protéines actives sur le plan biologique à des températures augmentées par mise en contact de ces dernières avec une combinaison de chaperons isolés passifs et actifs provenant d'un eukariote hyperthermophile, ces chaperons pouvant consister en des protéines de choc thermique, préfoldine et/ou chaperonine.

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