Recombinant poxvirus for chimeric proteins of the human...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/48 (2006.01) C07K 14/065 (2006.01) C07K 14/155 (2006.01) C07K 14/16 (2006.01) C12N 15/62 (2006.01) C12N 15/863 (2006.01)

Patent

CA 2430702

The invention relates to HIV chimeric genes formed by the union of fragments of different genes of said virus, wherein said fragments contain epitopes for cytotoxic T cells (CTL) or HIV-1 auxiliary T cells, which are presented by a wide range of antigens of type 1 Major Human Histocompatibility System (HLA- 1). Recombinant poxvirus are obtained from said genes, which are useful for prophylactic and therapeutic vaccination against HIV/AIDS infection, are capable of generating a protective immune cell response in vaccinated laboratory animals and are recognized by the CTL lymphocytes of HIV/AIDS patients.

L'invention concerne des gènes chimères du VIH formés à l'aide de fragments de différents gènes de ce virus, ces fragments renfermant des épitopes pour des lymphocytes T cytotoxiques (CTL) ou T auxiliaires du VIH-1, représentés par une gamme étendue d'antigènes du système majeur d'histocompatibilité humain de type 1 (HLA-1). On obtient à partir de ces gènes des poxvirus recombinés utiles pour la vaccination préventive et thérapeutique contre l'infection par le VIH/SIDA, permettant de générer une réponse immune cellulaire protectrice chez des animaux de laboratoire immunisés et reconnus par les lymphocytes CTL de patients atteints par le VIH/SIDA.

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