C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/34 (2006.01) C12Q 1/02 (2006.01) G01N 21/64 (2006.01) G01N 33/497 (2006.01)
Patent
CA 2147561
A culture medium and blood specimen are introduced into a sealable glass vial having a headspace gas mixture such that a change in the gas mixture composition can be monitored by a chemically sensitive material in the vial comprising a mixture of a fluorophore and a chromophore. The fluorophore exhibits a long fluorescence decay time and a fluorescence intensity that depend on a first chemical parameter, such as oxygen concentration. The chromophore exhibits an optical transmission that depends on a second chemical parameter, such as carbon dioxide concentration, the optical transmission of the chromophore changing with the second chemical parameter either within the excitation or within the emission wavelength range of the fluorophore.
n milieu de culture et un échantillon de sang sont introduits dans une fiole en verre étanche contenant un matériel chimiosensible composé d'un mélange d'un fluorophore et d'un chromophore. Ce matériel permet de mesurer les changements de la composition du mélange gazeux qui occupe l'espace libre. Le fluorophore est caractérisé par une longue durée d'extinction de la fluorescence, et l'intensité de la fluorescence dépend d'un premier paramètre chimique, la concentration d'oxygène. La transmission optique du chromophore varie en fonction d'un deuxième paramètre chimique, la concentration de dioxyde de carbone, à l'intérieur de la gamme de longueurs d'onde d'excitation ou d'émission du fluorophore.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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