Method and apparatus for speciating and identifying mai...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) C12M 1/14 (2006.01) C12Q 1/18 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2069889

2069889 9106669 PCTABS00005 A method of speciating and identifying MAI in a specimen comprises placing a paraffin coated slide in a receptacle containing a sterile aqueous solution inoculated with the specimen, analyzing the slide after exposure to the specimen to determine the presence or absence of atypical Mycobacteria, and after the analysis step, if atypical Mycobacteria are determined to be present, performing at least one speciation assay to ascertain if the atypical Mycobacteria are MAI. A related apparatus is also disclosed for speciating and identifying MAI in a specimen comprising a paraffin-wax coated slide, a tube having a sterile aqueous solution contained therein, the tube adapted to hold the slide, and at least one speciation assay means for performing an assay to determine the presence or absence of MAI in the speciment after the speciment is introduced into the tube holding the solution and the slide. An apparatus and method for determining the sensitivity of MAI to different antibiotics and dosages thereof is also provided.

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