A method for detecting conformational change of calmodulin,...

Details A method for detecting conformational change of calmodulin,... A method for detecting conformational change of calmodulin,...

G - Physics

01

N

G01N 19/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2586415

A method which can detect a calmodulin structure change easily and quantitatively in a short time and in real time, and a method which can retrieve effectively a matter having activity affecting a calmodulin structure change from among many matters. A matter under test from which activity is to be detected is allowed to act on a sample film consisting of calmodulin to detect changes in sample film tension and/or elasticity made after the matter under test is acted on by a mechanochemical sensor, whereby the method can measure, in real time and in a short time, activity that induces or blocks a calmodulin structure change provided by the matter under test.

L'invention concerne un procédé capable de détecter un changement de structure de calmoduline facilement et quantitativement en un court laps de temps et en temps réel, et un procédé capable de récupérer de manière efficace une matière ayant une activité affectant un changement de structure de calmoduline parmi de nombreuses matières. On laisse une matière soumise à l'essai, dont on cherche à détecter l'activité, agir sur un film échantillon consistant en calmoduline pour détecter des changements de tension et/ou d'élasticité de film échantillon apportés une fois que la matière en cours d'analyse a subi l'action d'un capteur mécanochimique. Le procédé peut mesurer, en temps réel et en un court laps de temps, une activité induisant ou bloquant un changement de structure de calmoduline provoqué par la matière en cours d'analyse.

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