Nucleic acids extension assay

Details Nucleic acids extension assay Nucleic acids extension assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2118913

Disclosed is a method of testing for the presence of a nucleic acid se-quence of interest in a sample, comprising: contacting the sample with a first probe and a second probe, portions of said probes being capable of hybridis- ing to the sequence of interest such that the probes are adjacent or substan- tially adjacent to one another, so as to enable other portions of the first and second probes to become annealed to each other: treating the sample so as to cause chain extension of one of the probes using part of a probe as a tem-plate; and detecting at least part of the extended probe.

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