Method of cryopreserving cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/07 (2010.01) C12N 1/04 (2006.01) C12N 5/00 (2006.01) G06F 19/00 (2011.01) A01N 1/02 (2006.01)

Patent

CA 2482045

A non-linear cryopreservation protocol and method for improving cryopreservation protocols for cells involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cryopreservation protocol for cryopreserving stem cells is also diclosed.

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