Process for immobilizing enzymes to the cell wall of a...

Details Process for immobilizing enzymes to the cell wall of a... Process for immobilizing enzymes to the cell wall of a...

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/395 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 9/20 (2006.01) C12N 9/40 (2006.01) C12N 11/16 (2006.01) C12N 15/53 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2139670

A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokariotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.

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