Nucleotide sequences and methods for detection of serpulina...

Details Nucleotide sequences and methods for detection of serpulina... Nucleotide sequences and methods for detection of serpulina...

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07H 21/04 (2006.01) C07K 14/20 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2125120

The invention provides a method for detecting the presence of Serpulina hyodysenteriae in a biological sample, an oligonucleotide primer and an S. hyodysenteriae- specific oligonucleotide probe useful in that method, and an article of manufacture that contains the primers and/or probe. Also provided are an about 2.3-kb DNA fragment derived from genomic DNA of S. hyodysenteriae and encoding for an about 56 kDa polypeptide, a recombinant expression vector containing the DNA fragment, the 56 kDa polypeptide and a monoclonal antibody reactive with the peptide, and a method of assaying for antibodies reactive with the 56 kDa peptide.

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