Inhibition of stem cell differentiation, enhancement of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/0775 (2010.01) C12N 5/071 (2010.01) A61K 35/42 (2006.01) A61K 38/18 (2006.01) C07K 14/475 (2006.01)

Patent

CA 2545520

The invention relates to a method for at least in part inhibiting differentiation of stem cells in a population of mammalian cells comprising upregulating a Wnt signaling pathway to a differentiation inhibiting level in said population of cells. The invention increases at the same time the number of stem cells in a population of mammalian cells compared to a reference population, and induces at least in part apoptosis in mesenchymal cell in a population of mammalian cells The invention also discloses a method for selective differentiation of a stem cell, comprising controlling the level of Wnt pathway activation. The invention is used for the proliferation and subsequent differentiation of embryonic stem cells and lung stem cells, and for ex vivo lung explant cultivation.

L'invention concerne un procédé destiné à inhiber au moins en partie la différentiation des cellules souches dans une population de cellules mammaliennes, y compris la régulation vers le haut de la voie de signalisation Wnt jusqu'à un niveau d'inhibition de la différentiation dans ladite population de cellules. L'invention permet d'augmenter simultanément le nombre de cellules souches dans une population de cellules mammaliennes en comparaison à une population de référence et induit une apoptose au moins partielle dans une cellule mésenchymale d'une population de cellules mammaliennes. L'invention concerne aussi un procédé destiné à la différentiation sélective d'une cellule souche, qui consiste à réguler le degré d'activation de la voie de signalisation Wnt. L'invention s'utilise dans la prolifération et, par la suite, la différentiation des cellules souches embryonnaires et des cellules souches du poumon, et dans la culture ex vivo d'explants du poumon.

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