Vitamin c production in microorganisms and plants

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/00 (2006.01) C12N 1/12 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 5/04 (2006.01) C12N 9/90 (2006.01) C12N 15/82 (2006.01) C12P 17/04 (2006.01)

Patent

CA 2331198

A biosynthetic method for producing vitamin C (ascorbic acid, L-ascorbic acid, or AA) is disclosed. Such a method includes fermentation of a genetically modified microorganism or plant to produce L-ascorbic acid. In particular, the present invention relates to the use of microorganisms and plants having at least one genetic modification to increase the action of an enzyme involved in the ascorbic acid biosynthetic pathway. Included is the use of nucleotide sequences encoding epimerases, including the endogenous GDP-D-mannose:GDP-L- galactose epimerase from the L-ascorbic acid pathway and homologues thereof for the purposes of improving the biosynthetic production of ascorbic acid. The present invention also relates to genetically modified microorganisms, such as strains of microalgae, bacteria and yeast useful for producing L- ascorbic acid, and to genetically modified plants, useful for producing consumable plant food products.

L'invention concerne une méthode biosynthétique de production de vitamine C (acide ascorbique, acide L-ascorbique ou AA). Cette méthode consiste en la fermentation d'un micro-organisme ou d'une plante génétiquement modifiée pour produire de l'acide L-ascorbique. En particulier, la présente invention concerne l'utilisation de micro-organismes et de plantes ayant au moins une modification génétique pour augmenter l'action d'une enzyme impliquée dans la voie biosynthétique de l'acide ascorbique. On a également prévu l'utilisation de séquences nucléotidiques codant des épimérases, notamment le GDP-D-manose endogène: GDP-L-galactose épimérase de la voie de l'acide L-ascorbique et ses homologues aux fins d'améliorer la production biosynthétique d'acide ascorbique. La présente invention concerne également des micro-organismes génétiquement modifiés, tels que des souches de micro-algues, de bactéries et de levures utiles pour la production d'acide L-ascorbique, ainsi que des plantes génétiquement modifiées, utiles pour la production de produits alimentaires végétaux consommables.

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