C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) A61B 10/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2143053
Disclosed is a method of evaluating the in vitro or in vivo fertilization capability of a given sperm sample. The method involves the steps of capacitating a sample of mammalian sperm cells followed by incubating the capacitated sperm with a predetermined amount of a glycoside-macromolecule conjugate such as a neoglycoprotein or a glycodendrimer for a period of time and under conditions sufficient to induce an acrosome reaction in the sperm. This step effectively mimics the physiological reaction that occurs in vivo between a sperm and an egg. The extent of the conjugate-induced acrosome reaction in the sperm cells relative to a positive control and a negative control, which account for the percent of sperm in the sample that acrosome react non-physiologically and spontaneously, respectively, is then calculated. The fertilization capability of the sperm is then evaluated as a function of the extent of this calculation. Also disclosed are kits which contain the necessary materials for conducting the method in a clinical laboratory.
Brandelli Adriano
Tezon Jorge G.
Fundacion Instituto de Biologia Y. Medicina Experimental
Moffat & Co.
LandOfFree
Induction of the acrosome reaction to evaluate the... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Induction of the acrosome reaction to evaluate the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Induction of the acrosome reaction to evaluate the... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1609127