Method for the isolation of spiroplasma in mammals

Details Method for the isolation of spiroplasma in mammals Method for the isolation of spiroplasma in mammals

A - Human Necessities

61

K

A61K 39/00 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2607170

The present invention is directed to a method of isolating and culturing spiroplasma by obtaining a tissue sample from a bovine; inoculating embryonated eggs with the tissue sample; mixing fluid from the inoculated egg with culture media; culturing the media mixed with the egg fluid at approximately 32~C for preferably greater than a month to establish a spiroplasma culture.

L'invention concerne un procédé d'isolement et de culture de spiroplasmes par prélèvement d'un échantillon de tissus sur un bovin ; par inoculation d'oeufs embryonnaires avec l'échantillon de tissus ; par mélange du fluide issu de l'oeuf inoculé avec le milieu de culture ; par culture du milieu mélangé au fluide de l'oeuf à une température avoisinant 32 °C pendant une durée supérieure à un mois de préférence de manière à créer une culture de spiroplasmes.

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