Regulatory constructs comprising intron 3 of prostate...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C07K 14/435 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2364492

The invention provides regulatory constructs comprising intron 3 of the prostate specific membrane antigen gene (PSMA). An isolated nucleic acid molecule encoding the partial sequence of intron 3 of PSMA, a vector and a recombinant expression cassette are disclosed. The invention also provides a method of directing expression of a coding sequence in a prostate cell, a bladder cell, a breast cell and a vascular endothelial cell using the said constructs. This invention further provides a method of treatment of cancer using the said constructs.

L'invention concerne des produits de recombinaison régulateurs comprenant l'intron 3 du gène PSMA (antigène d'enveloppe prostatique spécifique). Cette invention concerne également une molécule d'acide nucléique isolée codant pour la séquence partielle de l'intron 3 dudit gène PSMA, et un vecteur et une cassette d'expression recombinante. Cette invention concerne en outre une méthode pour diriger l'expression d'une séquence codante dans une cellule prostatique, une cellule de Zander, une cellule mammaire, et une cellule endothéliale vasculaire, à l'aide des produits de recombinaison susmentionnés. Enfin, l'invention concerne une méthode de traitement du cancer s'appuyant sur l'utilisation de ces produits de recombinaison.

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