Soluble recombinant influenza antigens

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 39/145 (2006.01) A61K 39/385 (2006.01) C07K 14/11 (2006.01) C07K 19/00 (2006.01) C12N 15/44 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2730171

The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemag-glutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

L'invention porte sur une protéine hémagglutinine trimère recombinée (rHA) soluble comprenant un ectodomaine d'hémagglutinine et un domaine d'oligomérisation. La rHA est produite sous forme d'un homotrimère soluble et peut en outre comprendre un peptide signal et/ou un signal de rétention dans le réticulum endoplasmique (ER). L'invention porte également sur des acides nucléiques codant pour la rHA de l'invention, ainsi que sur des vecteurs et des produits de recombinaison chimères comprenant l'acide nucléique. L'invention porte également sur des procédés de fabrication de la rHA. La rHA décrite présentement peut être utilisée pour formuler des vaccins contre la grippe ou peut être utilisée pour enrichir des vaccins existants.

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