Nucleic acid probes and protein probes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) A61K 39/395 (2006.01) C07H 21/00 (2006.01) C12N 5/20 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/532 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2059559

2059559 9119729 PCTABS00008 Nucleic acid probes and protein probes are disclosed. The nucleic acid probe comprises a probe polynucleotide, a charged hapten label, and a binding moiety. The protein probe comprises a probe protein, a charged hapten label and a binding moiety. The charged hpaten label joined to the binding moiety can comprise a negatively charged sulfophenylhydrazine tag compound. Polyclonal antibodies and monoclonal antibodies with specific affinity for the charged hapten labels are disclosed as are hybridomas capable of making the monoclonal antibodies. Methods and kits are disclosed for making the nucleic acid probes, making the protein probes, detecting a capture polynucleotide of the nucleic acid probe and detecting a capture molecule of the protein probe.

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