Multi-wavelength fluorometric system for on-line monitoring...

G - Physics – 01 – N

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G01N 21/64 (2006.01) G01J 3/10 (2006.01)

Patent

CA 2575118

A fluorometric system for on-line monitoring of biological processes uses a plurality of light sources, each with a different spectral width, to illuminate a sample, the light sources selected to produce emission of fluorescent light, and usually also dispersed light, from the illuminated sample. One of the light sources has a wide spectral range. The light sources are operated sequentially or in combination. Spectra acquired from the emitted light are combined and processed to evaluate properties of the sample, such as concentration of the fluorescing components or particle concentration.

L'invention concerne un système fluorométrique destiné à la surveillance en ligne de procédés biologiques et mettant en oeuvre une pluralité de sources lumineuses possédant individuellement une largeur spectrale distincte, aux fins d'éclairage d'un échantillon, les sources lumineuses étant sélectionnées aux fins de production d'une émission de rayonnement fluorescent et généralement également de rayonnement dispersé à partir de l'échantillon éclairé. Une des sources lumineuses comprend une large gamme spectrale. Les sources lumineuses sont mises en oeuvre de manière séquentielle ou conjointe. Les spectres acquis à partir du rayonnement émis sont combinés et traités, de manière à évaluer les propriétés de l'échantillon, telles que la concentration en composants fluorescents ou la concentration particulaire.

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