Secretion leader trap cloning method

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/66 (2006.01) C12N 15/10 (2006.01) C12N 15/18 (2006.01) C12N 15/62 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2223155

Expression vectors and methods for cloning amino-terminal signal sequences are disclosed. These vectors and methods are useful tools for selecting, expressing and isolating previously unknown signal sequences. In turn, an orphan signal sequence can be used to clone, identify and isolate the corresponding orphan proteins that the orphan signal sequence directs to the surface of a cell. Using these expression vectors and methods, an orphan protein can be cloned in the absence of a functional assay that detects such protein.

L'invention concerne des vecteurs d'expression et des procédés pour cloner des séquences N-terminales. Ces vecteurs et ces procédés sont utiles pour sélectionner, exprimer et isoler des séquences signal précédemment inconnues. A son tour, une séquence signal orpheline peut être utilisée pour cloner, identifier et isoler les protéines orphelines correspondantes que la séquence signal orpheline dirige vers la surface d'une cellule. En utilisant ces vecteurs d'expression et ces procédés, on peut cloner une protéine orpheline en l'absence d'un test d'activité détectant une telle protéine.

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