Method and device for concentrating selected groups of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/02 (2006.01) B01J 8/18 (2006.01) C12Q 1/04 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2342108

A method and device are described to concentrate target organisms from a mixture of organisms. Beads (1) made of material such as nylon, polystyrene or glass are coated with antibodies of specific microorganisms. The beads (1) are contained in an enclosure (2) surrounded by grid material (4). The pore size of the grid is smaller than the size of the beads, to assure that the beads stay within the grid material and larger than the size of the microorganisms to allow the interaction of the microorganisms with the beads. A rod (5) is attached to the upper part of the enclosure (2) allowing the agitation of the device inside he growth medium containing the target organisms.

La présente invention concerne un procédé et un dispositif permettant de concentrer des organismes cibles à partir d'un mélange d'organismes. Des perles (1) fabriquées dans une matière telle que le nylon, le polystyrène ou le verre sont recouvertes d'anticorps de micro-organismes spécifiques. Ces perles (1) sont contenues dans une enceinte (2) entourée d'un matériau grillagée. La taille des pores de la grille est inférieure à la taille des perles afin de s'assurer que les perles restent à l'intérieur du matériau grillagé, et plus grande que la taille des micro-organismes pour permettre l'interaction des micro-organismes avec les perles. Une ficelle (5) est attachée à la partie supérieure de l'enceinte (2) pour permettre d'agiter le dispositif dans le milieu de croissance contenant les organismes cibles.

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