Processes using dual specificity oligonucleotide and dual...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2599647

The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.

L'invention concerne plusieurs procédés effectués par réaction d'extension dépendante d'un modèle faisant intervenir un oligonucléotide à double spécificité et un oligonucléotide à double spécificité composé de trois parties Tm différentes utilisé dans lesdits procédés. L'invention concerne les caractéristiques dudit oligonucléotide à double spécificité, à savoir une haute spécificité d'hybridation et une tolérance au mésappariement élevée.

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