Recombinant expression vectors and purification methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/24 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2071196

Recombinant DNA sequences encoding the DNA polymerase I activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity, T. thermophilus DNA polymerase I is an ~ 94 kDa protein especially useful in the DNA amplification procedure known as the polymerase chain reaction.

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