A host cell comprising a vector for production of proteins...

Details A host cell comprising a vector for production of proteins... A host cell comprising a vector for production of proteins...

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) C12N 15/67 (2006.01) C12N 9/04 (2006.01) C12N 15/57 (2006.01)

Patent

CA 2602793

The present invention relates a host cell comprising an expression vector comprising a nucleic acid molecule encoding a protein requiring gamma- carboxylation and associated expression control sequences and a nucleic acid molecule encoding a vitamin K epoxido reductase and associated expression control sequences and a nucleic acid molecule encoding a .gamma.-glutamyl carboxylase and associated control sequences. The invention further relates to a method of producing a protein requiring gamma-carboxylation in high yields.

La présente invention concerne une cellule hôte comprenant un vecteur d'expression à molécule d'acide nucléique codant une protéine demandant une gamma-carboxylation et les séquences de contrôle d'expression associées, et une molécule d'acide nucléique codant une époxido-réductase de vitamine K et les séquences de contrôle d'expression associées, et une molécule d'acide nucléique codant une gamma-glutamyl-carboxylase et les séquences de contrôle associées. L'invention concerne également un procédé de production d'une protéine demandant une gamma-carboxylation à hauts rendements.

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