Compositions and methods for stem cell expansion and...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/02 (2006.01) A61K 35/12 (2006.01) A61L 27/14 (2006.01) A61L 27/38 (2006.01) C12N 5/10 (2006.01) C12N 11/02 (2006.01) C12N 11/04 (2006.01) A61L 27/58 (2006.01)

Patent

CA 2580754

The present invention relates to compositions comprising stem cells and partially committed progenitor cells and to methods of controlling cell proliferation and differentiation, which can be used for expansion of stem cells and their subsequent differentiation. The present invention provides expanded population of essentially undifferentiated stem cells, which are useful in clinical procedures involving stem cell therapy, and a population derived thereof of which at least part of the cells are differentiated. The cells can be used per se, as a part of a cell-bearing composition comprising cross-linked hyaluronic acid-laminin gels or as a part of a composite implant for tissue regeneration.

L'invention concerne des compositions contenant des cellules souches et des cellules précurseurs partiellement déterminées, ainsi que des méthodes servant à contrôler la prolifération et la différenciation cellulaires, qu'on peut mettre en application pour l'expansion de cellules souches et leur différenciation ultérieure. Elle concerne une population étendue de cellules souches essentiellement indifférenciées qui sont utiles dans des procédures cliniques impliquant une thérapie par les cellules souches, et une population qui en est dérivée dont au moins une partie des cellules est différenciée. On peut utiliser ces cellules per se, en tant que parties d'une composition contenant des cellules et comprenant des gels réticulés d'acide hyaluronique-laminine ou en tant que partie d'un implant composite permettant d'effectuer une régénération tissulaire.

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