C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/867 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2566423
The invention relates to HIV-based recombinant viral clones having the general structure shown in the accompanying figure, which are the result of the following genetic manipulations: deletion of HIV fragments (e.g. the Nef gene) without loss of infective capacity; insertion of a gene that is not expressed in human cells; insertion of the LacZ gene; and introduction of restriction sites, in order to extract DNA fragments from the matrix provirus and substitute same with genes from the patients to be assessed. In addition, the invention relates to the application of said clones in analytical methods that are associated with AIDS.
La présente invention concerne des clones viraux recombinés basés sur le VIH lesquels possèdent la structure générale représentée par la figure et sont le résultat des manipulations génétiques suivantes: délétion de fragments de VIH (par exemple, gène Nef) sans perte de capacité d'infection, insertion d'un gène non exprimé dans des cellules humaines, insertion du gène Lacz, introduction de sites de restriction pour extraire des fragments d'ADN du provirus matriciel et pour les remplacer par des gènes de patients à évaluer. De même, l'invention concerne l'application desdits clones à des méthodes analytiques concernant le SIDA.
Alcami Pertejo Jose
Garcia Perez Javier
Gonzalez Fernandez Nuria
Sanchez Palomino Sonsoles
Fundacion Para La Investigacion Y. La Prevencion Del Sida En Esp
Instituto de Salud Carlos III
Marks & Clerk
LandOfFree
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