Method for the preservation of viruses and mycoplasma

Details Method for the preservation of viruses and mycoplasma Method for the preservation of viruses and mycoplasma

C - Chemistry, Metallurgy

12

N

C12N 1/04 (2006.01) A61K 39/12 (2006.01) A61K 39/13 (2006.01) A61K 39/155 (2006.01) A61K 39/165 (2006.01) A61K 39/17 (2006.01) A61K 39/20 (2006.01) C12N 7/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2365277

A biologically-active material comprising a live virus or mycoplasma is preserved by a method of desiccation, without lyophilisation, in a matrix of glassy trehalose having a residual moisture content of not greater than 2 %. The method comprises two vacuum drying stages. In a cycle time much shorter than a typical freeze drying process a virus or mycoplasma can be preserved to provide a material that can be rehydrated to give a vaccine having potency.

Selon l'invention, un matériau biologiquement actif contenant un virus ou un mycoplasme actif est conservé par un procédé de dessiccation, sans lyophilisation, dans une matrice de tréhalose vitreuse présentant un pourcentage d'humidité résiduelle inférieur à 2 %. Le procédé comprend deux étapes de dessiccation sous vide. Pendant une durée de cycle beaucoup plus courte que celle d'un processus de cryodessiccation classique, un virus ou mycoplasme peut être conservé de manière à former un matériau pouvant être réhydraté pour l'élaboration d'un vaccin efficace.

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