Position-specific insertion vectors and method of using same

Details Position-specific insertion vectors and method of using same Position-specific insertion vectors and method of using same

C - Chemistry, Metallurgy

12

N

195/1.111, 195/1

C12N 15/00 (2006.01) C07K 16/40 (2006.01) C12N 5/00 (2006.01) C12N 9/22 (2006.01) C12N 15/81 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2003695

-37- POSITION-SPECIFIC INSERTION VECTORS AND METHOD OF USING SAME ABSTRACT OF THE DISCLOSURE The present invention provides vectors for the efficient and position-specific integration of expressible, exogenous nucleotide sequences into cellular genomes. This invention takes advantage of the discovery of a position- specific endonuclease and position-specific insertion markers for the design of said vectors. In addition, a gene comprising a recombinant nucleic acid molecule encoding a polypeptide possessing the biological activity of a position-specific endonuclease, wherein the biological activity of said endonuclease is the catalysis of position- specific insertion of genetic material carried between the position-specific integration markers, is disclosed, UCALFO8A.PCT 110889 -37-

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