Method and apparatus for screening microscopic cells in aged...

G - Physics – 01 – N

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G01N 33/49 (2006.01) G01N 1/34 (2006.01) G01N 15/12 (2006.01) G01N 15/14 (2006.01) G01N 33/50 (2006.01) G01N 33/553 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 15/10 (2006.01)

Patent

CA 2102243

ABSTRACT A method and apparatus for automatically retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of an aged whole blood sample or portion thereof. A volume of a biological sample containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed. The total white blood cell populations and lymphocyte population are first electronically counted and then the neutrophil population including the aged neutrophil population is removed from the same or a second sample portion. The opacity and/or volume parameter of the cells then can be modified and the mixture then again counted and analyzed to obtain the desired white blood cell population analysis.

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