Human papillomavirus detection assay

Details Human papillomavirus detection assay Human papillomavirus detection assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07H 21/00 (2006.01) C12N 11/10 (2006.01)

Patent

CA 2139623

A two-step nucleic acid hybridization probe assay for certain types of human papilloma virus (HPV) associated with cervical cell dysplasia and malignancy comprises a fluid phase capture hybridization step in which amplified specific gene E6/E7 messenger RNA from a biological specimen is hybridized to a biotinylated capture reagent to form a complex, attachment of the capture reagent complex to a solid phase by reaction with immobilized streptavidin, a second hybridization step in which a virus type-specific enzyme-conjugated detection probe hybridizes with the complexed amplified messenger RNA, and detection of the complexed detection probe by color or fluorophor production following a wash of the solid phase and addition of an appropriate chromogenic or fluorogenic substrate. The assay has enhanced sensitivity compared to conventional tests and is specific for actual expression of HPV oncogenes in cervical specimens, and not merely indicative of viral presence.

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