Methods of genetic analysis involving the amplification of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G06F 17/16 (2006.01)

Patent

CA 2597947

The invention relates to methods for genetic analysis of DNA sequences containing complementary duplicons which are duplicated and linked DNA sequences separated by an intermediate sequence. Furthermore, the invention relates to the Bidirectional Amplification of Complementary Duplicons (BACD) using a single primer. The amplification of complementary duplicons can be used for a variety of purposes such as, but not limited to, determining the species from which a sample comprising genomic DNA is derived, or used as a marker for a trait of interest. Also provided are means for analysing large datasets which can be used to identify primers useful for the genetic analysis methods of the invention.

L'invention concerne des procédés pour l'analyse génétique de séquences d'ADN contenant des duplicons complémentaires lesquels sont des séquences d'ADN dupliquées et liées séparées par une séquence intermédiaire. En outre, l'invention concerne l'amplification bidirectionnelle de duplicons complémentaires (BACD) en utilisant une seule amorce. On peut utiliser l'amplification de duplicons complémentaires à diverses fins telles que, mais elles ne sont pas limitées à celles-ci, la détermination des espèces à partir desquelles est dérivé un échantillon comprenant de l'ADN génomique, ou bien on peut les utiliser comme marqueur pour un trait qui présente un intérêt. L'invention concerne également des moyens servant à analyser de grands ensembles de données qu'on peut utiliser pour identifier des amorces utiles pour les procédés d'analyse génétique de l'invention.

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