Continuous fermentation process

Details Continuous fermentation process Continuous fermentation process

C - Chemistry, Metallurgy

12

P

C12P 7/60 (2006.01)

Patent

CA 2277539

A process for the continuous production of 2-keto-L-gulonic acid or a salt thereof from D-sorbitol by fermentation with microorganisms is characterized in that a nutrient medium containing D-sorbitol is incubated in a first fermentation vessel with a microorganism capable of converting D-sorbitol to L-sorbose, whereafter the resulting fermentation broth containing L-sorbose is transferred to a second fermentation vessel where it is incubated with a microorganism capable of converting L-sorbose to 2-keto-L-gulonic acid. In a particularly preferred embodiment of this process the fermentation broth from the first fermentation vessel is sterilized before being transferred to the second fermentation vessel. 2-keto-L-gulonic acid is a valuable intermediate for the production of vitamin C.

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