Method op assessing the viability of thawed cells

Details Method op assessing the viability of thawed cells Method op assessing the viability of thawed cells

G - Physics

01

N

G01N 33/50 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2640116

The invention relates to a method of assessing the viability of a thawed cell wherein the cell is a gamete, an embryo, a karyoplast, a putative stem cell population, a stem cell precursor population or a stem cell population. The method comprises incubating the thawed cell in a culture medium including a plurality of amino acids and determining the change in concentration in the medium of at least one amino acid.

L'invention concerne un procédé d'évaluation de la viabilité d'une cellule décongelée, cette cellule étant un gamète, un embryon, un caryoplaste, une population de cellules souches putatives, une population de précurseurs de cellules souches ou une population de cellules souches. Le procédé comprend l'incubation de la cellule décongelée dans un milieu de culture contenant une pluralité d'acides aminés et la détermination de la variation de la concentration d'au moins un acide aminé dans le milieu.

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