Method for influencing the content of sinapine in transgenic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/06 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 9/10 (2006.01) C12N 15/29 (2006.01) C12N 15/56 (2006.01)

Patent

CA 2415554

The invention relates to a method for influencing the content of sinapine in transgenic plant cells and plants. The invention in particular relates to the inhibition of the enzymatic activity of a UDP-glucose: sinapoyl glucose transferase (SGT) in transgenic plant cells. The invention further relates to nucleic acid molecules containing a DNA sequence which codes for a protein with the enzymatic activity of an SGT. The invention furthermore relates to transgenic plants and plant cells, comprising the above nucleic acid molecules, as a result of which the above contain a reduced sinapine content in comparison with wild type plants or cells and the agricultural products and propagating material of the transgenic plants.

L'invention concerne un procédé permettant d'influencer la teneur en sinapine de plantes et cellules végétales transgéniques. Cette invention se rapporte en particulier à l'inhibition de l'activité enzymatique d'une UDP-glucose:acide sinapique glucosyle transférase (SGT) dans des cellules végétales transgéniques. L'invention concerne également des molécules d'acide nucléique qui contiennent une séquence d'ADN codant pour une protéine ayant l'activité enzymatique d'une SGT, des plantes et cellules végétales transgéniques qui contiennent une molécule d'acide nucléique selon ladite invention et présentent, de ce fait, une teneur réduite en sinapine par rapport à des plantes et cellules végétales sauvages, ainsi que des produits de récolte et le matériel génétique des plantes transgéniques.

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