Improved liquid chromatographic media for polynucleotide...

Details Improved liquid chromatographic media for polynucleotide... Improved liquid chromatographic media for polynucleotide...

B - Operations, Transporting

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B01D 15/08 (2006.01) B01J 20/26 (2006.01) B01J 20/32 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 30/88 (2006.01)

Patent

CA 2285307

Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped with a non- polar hydrocarbon or substituted hydrocarbon group. The beads provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography.

L'invention porte sur des billes non poreuses dont le diamètre moyen est compris entre environ 0,5 et 100 microns et qui sont appropriées pour être utilisées dans la séparation chromatographique. Ces billes comprennent des particules non poreuses qui sont recouvertes d'un polymère dont presque tous les groupes de la surface du substrat sont coiffés aux extrémités par un groupe d'hydrocarbures non polaires ou substitués. Les billes assurent une séparation efficace des polynucléotides par chromatographie polynucléotidique à compatibilité ionique.

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