Stable cell lines capable of expressing the adeno-associated...

Details Stable cell lines capable of expressing the adeno-associated... Stable cell lines capable of expressing the adeno-associated...

C - Chemistry, Metallurgy

12

N

C12N 7/01 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/86 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2176215

Stable packaging cell lines derived from human 293 cells which are transfected with an AAV vector having the AAV rep gene operably linked to a heterologous transcription promoter, such as the metallothionein promoter, or am AAV Rep78 insensitive homologous promoter and which are capable of producing AAV Rep proteins and being useful for packaging recombinant AAV vectors containing target polynucleotides.

Lignées cellulaires stables d'encapsidation dérivées de cellules 293 humaines transfectées avec un vecteur de virus adéno-associé (AAV) dans lequel le gène rep d'AAV est relié de manière fonctionnelle à un promoteur de transcription hétérologue tel que le promoteur de métallothionéine, ou un promoteur homologue insensible au Rep78 d'AAV. Ces lignées sont aptes à produire des protéines Rep d'AAV et conviennent à l'encapsidation de vecteurs d'AAV recombinés contenant des polynucléotides cibles.

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