Method of measuring glycoprotein

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2560104

It is intended to provide a method of measuring a glycoprotein, a glycopeptide or a glycoamino acid at a high accuracy while regulating the digesting effect of a protease, and a measurement reagent to be used in this measuring method. A method of measuring a glycoprotein, a glycopeptide or a glycoamino acid which comprises treating a glycoprotein-containing sample with a protease, treating the glycopeptide or the glycoamino acid thus liberated with a corresponding oxidase, and measuring the thus formed hydrogen peroxide by using peroxidase and an reagent undergoing color-development when oxidized, characterized in that the liquid reaction mixture before the treatment with the oxidase is regulated to pH 1 to 5; and a reagent for measuring the same.

L'objectif est de fournir une méthode permettant la mesure précise des glycoprotéines, glycopeptides ou acides glycoaminés tout en régulant la fonction de digestion de la protéase, ainsi que de fournir un réactif pouvant être utilisé pour cette méthode de mesure. Une méthode de mesure des glycoprotéines, glycopeptides ou acides glycoaminés qui comprend le traitement des échantillons contenant des glycoprotéines avec une protéase, le traitement des glycopeptides ou des acides glycoaminés libérés avec l'oxydase correspondante et la mesure du peroxyde d'oxygène formé à l'aide d'une peroxydase et d'un indicateur coloré réagissant en cas d'oxydation, qui se caractérise par le fait que le mélange réactif avant traitement par oxydase présente un pH de 1 à 5 ; et un réactif pour mesurer ce dernier.

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