Assay for microorganisms and device for use therein

Details Assay for microorganisms and device for use therein Assay for microorganisms and device for use therein

C - Chemistry, Metallurgy

12

Q

C12Q 1/24 (2006.01) A01J 11/12 (2006.01) B01L 3/14 (2006.01) C07K 1/36 (2006.01) C12M 1/12 (2006.01) C12M 1/24 (2006.01) C12Q 1/04 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2236685

An assay device comprises a centrifugation tube (1) and, as a sliding fit therein, a less deep inner tube (2) whose base (4) has one or more apertures sufficiently large to allow the passage of particles. This device can be used in an assay for microorganisms in a liquid sample containing fatty material, which comprises centrifuging the sample and (if necessary) a clearing agent in the device, removing the inner tube containing the fatty material, removing at least substantially all of the liquid supernatant in the centrifugation tube, and determining the presence of ATP in the sedimented pellet.

Dispositif d'analyse constitué d'un tube de centrifugation (1) et d'un tube intérieur (2) moins profond venant s'adapter par coulissement à l'intérieur, dont la base (4) présente une ou plusieurs ouvertures suffisamment larges pour permettre le passage de particules. Ce dispositif peut être utilisé dans une recherche de micro-organismes dans un échantillon liquide contenant des matières grasses, consistant à centrifuger l'échantillon et (si nécessaire) un agent éclaircissant dans le dispositif, à retirer le tube intérieur contenant la matière grasse, à retirer au moins sensiblement l'ensemble du liquide surnageant dans le tube de centrifugation et à déterminer la présence d'ATP dans le sédiment.

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