Methods and kits for detection of prion diseases

Details Methods and kits for detection of prion diseases Methods and kits for detection of prion diseases

G - Physics

01

N

G01N 33/68 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2537968

The invention relates to diagnostic methods and kits for detecting transmissible spongiform encephalopathies (TSEs) such as BSE, scrapie, chronic wasting disease and related diseases in animals and humans. The invention provides a method for determining whether an aberrant prion protein is present or absent in a mammalian sample comprising the steps of preparing in an extraction buffer that comprises a surfactant and a detergent a homogenate from said sample, incubating said homogenate in diluted extraction buffer with a protease, incubating with a chaotropic agent and at least one washing buffer and detecting said aberrant prion protein.

L'invention se rapporte à des procédés et des kits diagnostiques de détection d'encéphalopathies spongiformes transmissibles (EST) telles que l'encéphalopathie spongiforme bovine (ESB), la tremblante du mouton, l'encéphalopathie des cervidés et les maladies associées chez les animaux et les humains. Cette invention porte aussi sur un procédé permettant de déterminer si une protéine prion aberrante est présente ou absente dans un échantillon de mammifère. Ce procédé consiste à préparer un tampon d'extraction qui contient un agent de surface, un détergeant et un homogénat prélevés dans l'échantillon, à incuber cet homogénat dans le tampon d'extraction dilué avec une protéase, à incuber avec un agent chaotropique et au moins un tampon de lavage et à détecter cette protéine prion aberrante.

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