Immunotherapy with in vitro-selected antigen-specific...

A - Human Necessities – 61 – K

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A61K 38/20 (2006.01) A01N 25/00 (2006.01) A01N 63/00 (2006.01) A61K 31/675 (2006.01) A61K 31/7076 (2006.01) A61K 35/12 (2006.01) A61K 35/26 (2006.01) A61K 35/28 (2006.01) A61P 35/00 (2006.01) A61P 35/04 (2006.01)

Patent

CA 2497552

A method of promoting the regression of a cancer in a mammal comprising: (i) administering to the mammal nonmyeloablative lymphodepleting chemotherapy, and (ii) subsequently administering: (a) autologous T-cells, which have been previously isolated, selected for highly avid recognition of an antigen of the cancer, the regression of which is to be promoted, and rapidly expanded in vitro only once, and, either concomitantly with the autologous T-cells or subsequently to the autologous T-cells, by the same route or a different route, a T-cell growth factor that promotes the growth and activation of the autologous T-cells, or (b) autologous T-cells, which have been previously isolated, selected for highly avid recognition of an antigen of the cancer, the regression of which is to be promoted, modified to express a T-cell growth factor that promotes the growth and activation of the autologous T-cells, and rapidly expanded in vitro only once, whereupon the regression of the cancer in the mammal is promoted.

L'invention porte sur un procédé visant à favoriser la régression d'un cancer chez un mammifère. Ce procédé consiste à: (i) administrer au mammifère une chimiothérapie non myéloablative appauvrissant les lymphocytes et (ii) administrer ensuite: (a) des lymphocytes T autologues qui ont été auparavant isolés, sélectionnés en vue d'une reconnaissance extrêmement vive d'un antigène du cancer dont on veut favoriser la régression, et dilatés rapidement in vitro une seule fois, et, soit de manière concomitante avec les lymphocytes T autologues ou ultérieurement à ceux-ci, par la même voie ou une voie différente, administrer un facteur de croissance des lymphocytes T qui favorise la croissance et l'activation des lymphocytes T autologues ou (b) administrer des lymphocytes T autologues qui ont été auparavant isolés, sélectionnés en vue d'une reconnaissance extrêmement vive d'un antigène du cancer dont on veut favoriser la régression, modifiés pour exprimer un facteur de croissance des lymphocytes T qui favorise la croissance et l'activation des lymphocytes T autologues, et dilatés rapidement in vitro une seule fois, favorisant ainsi la régression du cancer chez un mammifère.

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