Method for the production of recombinant proteins, plasmids...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/68 (2006.01) C07K 14/50 (2006.01) C12N 15/18 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2197804

Expression plasmids comprising a nucleic acid sequence of interest controlled by a T7 bacteriophage promoter and a stabilizing region comprising all or part of the <u>par</u>region of the RP4 plasmid or a derivative thereof. The invention also concerns the use of said plasmids in the production of recombinant products.

La présente invention concerne des plasmides d'expression comprenant une séquence d'acide nucléique d'intérêt sous le contrôle d'un promoteur du bactériophage T7 et une région stabilisatrice comprenant tout ou partie de la région par du plasmide RP4 ou d'un dérivé de celle-ci; et leur utilisation pour la production de produits recombinants.

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