Methods for enhancing the expression of a protein of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/66 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2407710

The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in eukaryotic cells. A strategy is provided for enhancing the synthesis of recombinant amino acid sequences by polymerizing expression cassettes in vitro before producing recombinant hosts.

L'utilisation accrue de techniques d'exploration de données concernant des séquences nucléotidiques a amplifié la demande de méthodes efficaces de production de protéines recombinées dans des cellules eucaryotes. L'invention concerne une stratégie visant à améliorer la synthèse de séquences d'acides aminés recombinées par polymérisation de cassettes d'expression in vitro avant de produire des hôtes recombinés.

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