C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/84 (2006.01) A01H 4/00 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2240454
A method by which Indica rice can be efficiently transformed. In this method, immature Indica rice germ cells are transformed by the Agrobacterium method and the resultant transformants are screened with the use of media containing 2,000 to 4,000 mg/l of KNO3, 60 to 200 mg/l of MgSO4, 200 to 600 mg/l of KH2 PO4, 100 to 450 mg/l of CaCl2, 200 to 600 mg/l of (NH4)2 SO4, 1 to 7 mg/l of H3BO3, 2 to 20 mg/l of MnSO4, 20 to 50 mg/l of EDTA or its salt, 3 to 8 mg/l of Fe, 50 to 200 mg/l of myoinositol, 0.5 to 10 mg/l of 2,4- dichloropheoxyacetic acid, 0.01 to 5 mg/l of cytokinins, 5,000 to 80,000 mg/l of saccharides and gelling agents and having a pH value of 4.5 to 6.5.
Procédé permettant de transformer efficacement du riz de type Indica. Selon ledit procédé, des cellules germinales de riz de type Indica immature sont transformées par le procédé des agrobactéries et les cellules transformées qui en résultent sont criblées à l'aide d'un milieu contenant 2.000 à 4.000 mg/l de KNO3, 60 à 200 mg/l de MgSO4, 200 à 600 mg/l de KH2PO4, 100 à 450 mg/l de CaCl2, 200 à 600 mg/l de (NH4)2SO4, 1 à 7 mg/l de H3BO3, 2 à 20 mg/l de MnSO4, 20 à 50 mg/l de EDTA ou son sel, 3 à 8 mg/l de Fe, 50 à 200 mg/l de myoinositol, 0,5 à 10 mg/l d'acide 2,4-dichlorophéoxyacétique, 0,01 à 5 mg/l de cytokinines, 5.000 à 80.000 mg/l de saccharides et d'agents gélifiants, et présentant une valeur pH de 4,5 à 6,5.
Hiei Yukoh
LandOfFree
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