Antifungal polypeptides

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/385 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01N 63/00 (2006.01) A01P 3/00 (2006.01) C07K 14/37 (2006.01) C12N 5/10 (2006.01) C12N 15/31 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2571369

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

L'invention concerne des compositions et des méthodes servant à protéger une plante contre un pathogène, en particulier, un pathogène fongique. Ces compositions comprennent de nouvelles séquences d'acides aminés, ainsi que leurs variantes et fragments, de polypeptides antipathogènes isolés de bouillon de fermentation microbienne. Elle concerne également des molécules d'acide nucléique comprenant des séquences de nucléotide codant pour ces polypeptides antipathogènes. Elle concerne, de plus, une méthode servant à induire la résistance contre le pathogène chez une plante au moyen de ces séquences de nucléotide. Cette méthode consiste à introduire dans une plante une cassette d'expression possédant un promoteur lié à une séquence de nucléotide codant pour un polypeptide antipathogène. Elle concerne également des compositions contenant un polypeptide antipathogène ou un micro-organisme transformé comprenant un acide nucléique combiné à un véhicule et des méthodes servant à utiliser ces compositions afin de protéger la plante contre le pathogène. Elle concerne enfin des plantes, des cellules de plante, des semences transformées et des micro-organismes comprenant une séquence de nucléotide codant pour le polypeptide antipathogène ou une de ses variantes ou fragments.

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