Methods and devices for diagnosis of appendicitis

C - Chemistry – Metallurgy – 40 – B

Patent

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C40B 40/10 (2006.01) C07K 14/47 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2678145

An immunoassay device and method is provided for determining the severity of appendicitis in a patient that includes testing a blood, serum or plasma sample from the patient for the quantity of MRP8/14 in the sample and comparing it with the quantity of MRP8/14 present in standard samples correlated with an appendicitis severity scoring system. Standard samples and data correlating MRP8/14 quantities present in patient samples to histologically-based appendicitis severity grades are also provided. The methods and immunoassay devices and kits of this invention are useful for managing the treatment of patients presenting with appendicitis symptoms.

L'invention porte sur un dispositif d'immunoessai et sur une méthode permettant de déterminer la sévérité d'une appendicite chez un patient, qui consiste à tester un échantillon de sang, de sérum ou de plasma d'un sujet pour y mesurer la quantité de MRP8/14 et la comparer à la quantité de MRP8/14 présente dans des échantillons standard corrélés avec un système de gradation de sévérités d'appendicites. L'invention porte également sur des échantillons standard et des données de corrélation des quantités de MRP8/14 présentes dans des échantillons de sujets avec des gradations de sévérités d'appendicites à base histologique. Les méthode, les dispositifs d'immunoessai et les trousses, de l'invention permettent de traiter des patients présentant des symptômes d'appendicite.

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